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pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 T18N

Plasmid

#47897

Purpose

Expresses myc tagged Miro2 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 T18N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 A13V

Plasmid

#47896

Purpose

Expresses myc tagged Miro2 A13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 A13V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

A13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-shC

Plasmid

#21963

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 E208K/E328K

Plasmid

#47900

Purpose

Expresses myc tagged Miro2 V208K/E328K mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 E208K/E328K (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

E208K/E328K, abolishes calcium binding

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-shD

Plasmid

#21964

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-H2BmCherry-OptoJNKi

Plasmid

#89741

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Optogenetics

Tags

Histone2B and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi

Plasmid

#89744

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

Plasmid

#229770

Purpose

Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Article

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)

Use

Luciferase

Tags

LgBiT

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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