We narrowed to 7,293 results for: ef1a

1. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

   Plasmid

   #193728

   Purpose

   Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

   Depositor

   Colin Akerman

   Article

   Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

   Insert

   ClopHensorN
   

   Use

   AAV

   Tags

   Strep-II (N terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a DIO-ChRmine-oScarlet-Kv2.1-WPRE

   Plasmid

   #183525

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   ChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a DIO-hsChRmine-oScarlet-Kv2.1-WPRE

   Plasmid

   #183527

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   hsChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H33R

   Promoter

   Ef1a

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

   Plasmid

   #170318

   Purpose

   Expresses FLAG and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

   Plasmid

   #183529

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   rsChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I146M/G174S

   Promoter

   Ef1a

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

   Plasmid

   #170319

   Purpose

   Expresses HA and EGFP tagged RPL22, neomycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

   Plasmid

   #170322

   Purpose

   Expresses FLAG and mCherry tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

    Plasmid

    #192203

    Purpose

    Lenti-PL(Puro-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-PL(Puro-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid

    #112677

    Purpose

    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Nuclear-localized floxed-mCherry EGFP
    

    Use

    AAV

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP

    Plasmid

    #61738

    Purpose

    Express Hoxa9 and MeisI to induce leukemic transformation. GFP label transduced cells.

    Depositor

    Yi Zhang

    Article

    He et al Blood. 2011 Apr 7;117(14):3869-80. doi:

    Insert

    Hoxa9-MeisI-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

    Plasmid

    #170317

    Purpose

    Expresses HA and EGFP tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

    Plasmid

    #170320

    Purpose

    Expresses HA and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

    Plasmid

    #216247

    Purpose

    Express OsTIR1.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    OsTIR1.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

    Plasmid

    #184631

    Purpose

    Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad65 (Gad2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge