We narrowed to 7,391 results for: ef1a;

1. pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

   Plasmid

   #139237

   Purpose

   mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

   Plasmid

   #139234

   Purpose

   mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

   Plasmid

   #139233

   Purpose

   mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

   Plasmid

   #139232

   Purpose

   mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

   Plasmid

   #139231

   Purpose

   mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

   Plasmid

   #128764

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #3 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

   Plasmid

   #128763

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #2 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

   Plasmid

   #128762

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #1 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

   Plasmid

   #128760

   Purpose

   Expresses PARN gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PARN sgRNA #2 (PARN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

    Plasmid

    #128758

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #3 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

    Plasmid

    #128756

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121803

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

    Plasmid

    #117137

    Purpose

    Expression vector encoding BFP-BSD-Nkx2-5

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

    Plasmid

    #198337

    Purpose

    PB plasmid expressing Halo-tagged PP7 coat protein

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    PP7 coat protein 3x Halo-GB1
    

    Use

    Piggybac

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid

    #233033

    Purpose

    To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d and mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge