We narrowed to 54,060 results for: tra

1. Arabidopsis thaliana Atm3 d80 pET21a+

   Plasmid

   #173045

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   with N-terminal 80 amino acids deletion

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BRD4 CRISPRi plasmid

   Plasmid

   #154890

   Purpose

   Human BRD4 CRISPRi gRNA

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   BRD4-targeting gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX4

   Plasmid

   #183903

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti PUb

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX3

   Plasmid

   #183904

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti Polyubiquitin

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-attB-PUb-GFP

   Plasmid

   #183911

   Purpose

   attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti PUb

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBCC091

   Plasmid

   #202218

   Purpose

   Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-LS

   Plasmid

   #205438

   Purpose

   Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

   Depositor

   Masamichi Kamihira

   Article

   Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

   Inserts

   Gaussia luciferase gene
   spectinomycin resistance gene
   

   Use

   Cre/Lox and Luciferase; Chlamydomonas expression

   Tags

   Expression

   Mutation

   Promoter

   Heat shock protein 70A (HSP70A)/ribulose bisphosp…

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKSB-AeU6-1gRNA

   Plasmid

   #183912

   Purpose

   gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti U6 promoter (AAEL017702)

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKSB-AeU6-2gRNA

    Plasmid

    #183913

    Purpose

    gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti U6 promoter (AAEL017774)

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAB1907

    Plasmid

    #47857

    Purpose

    BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    BBa_B1006 U10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added two extra U at the end of Bba_1006 poly-U t…

    Promoter

    pLTetO12

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Use

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS2155

    Plasmid

    #141065

    Purpose

    AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

    Insert

    SYN1-EmGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    April 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    MLV promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

    Plasmid

    #200283

    Purpose

    Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF1010

    Plasmid

    #143742

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF547 (ZNF547 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF0702

    Plasmid

    #144361

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOR2 (NCOR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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