Addgene

We narrowed to 31,164 results for: uros

Showing: 1481 - 1500 of 31164 results

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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Use

Tags

pEGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE puro PPAR delta

Plasmid

#8891

Depositor

Bruce Spiegelman

Article

Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

Insert

PPAR delta (Ppard Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Puromycin-mVenus-MAP

Plasmid

#44118

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-EGFP-FAK

Plasmid

#38194

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

Plasmid

#218529

Purpose

sgRNA targeting human EIF2AK1/HRI

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK1 (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH1-T2A-PuroR

Plasmid

#162342

Purpose

Lentiviral expression of ATOH1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

Plasmid

#176932

Purpose

Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-G266E

Plasmid

#225468

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

G266E

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-I304N

Plasmid

#225463

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-PSMD4

Plasmid

#202666

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a-ABCD3-RFP-Puro

Plasmid

#170168

Purpose

Mammalian Expression of Ef1a-ABCD3-RFP-Puro

Depositor

Christopher Moxham

Insert

ABCD3-RFP-Puro

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro_zhang2.0

Plasmid

#167919

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-puro

Plasmid

#51240

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-PuroR Xlone eGFP

Plasmid

#179837

Purpose

Doxycycline-inducible expression of eGFP

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN ; Donor plas…

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-FBL

Plasmid

#179235

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-puro/RAF1-S259A

Plasmid

#131727

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S259A

Promoter

CMV

Available since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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