164,599 results

1. pOSIP-TT (TetR, P21)

   Plasmid

   #45989

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR221_SLC26A5

   Plasmid

   #132044

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC26A5 (SLC26A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSC201plus

   Plasmid

   #164227

   Purpose

   Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

   Depositor

   Silvia Cardona

   Article

   Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Rhamnose-inducible

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 19 pCOLI_A_SUMO

   Plasmid

   #160257

   Purpose

   N-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pG1

   Plasmid

   #162602

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti X1 Puro DEST (694-6)

   Plasmid

   #17297

   Purpose

   3rd gen lentiviral promoter-less Gateway destination vector, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. APEX2-OMM

   Plasmid

   #79056

   Purpose

   Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Soybean APEX2
   

   Use

   Lentiviral

   Tags

   flag and last 31 amino acids of human MAVS

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiCRISPR v2 mBAX

    Plasmid

    #129577

    Purpose

    CRISPR deletion of murine BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAX (Bax Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge