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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3g-mNeon-WPRE-pA

Plasmid

#192064

Purpose

mNeonGreen expression with TRE3g promoter

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE3g

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AA068

Plasmid

#215994

Purpose

Fragmid fragment: (Selection) puromycin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; PuroR_v1.2

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV OMM-FKBP-V5-sTurboID (N)

Plasmid

#153006

Purpose

Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

OMM (Tom20)-sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-hAIM2

Plasmid

#51542

Purpose

Express GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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OpenCRISPR-1 sgRNA-12nt stem

Plasmid

#221567

Purpose

Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 12nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Murine cell surface protein knockout (McspKO) library

Pooled Library

#200445

Purpose

Knockout library targeting mouse cell surface genes.

Depositor

Junjie Chen

Article

Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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