We narrowed to 8,810 results for: Ott;

1. pRB14

   Plasmid

   #52522

   Purpose

   expresses a myc-tagged version of hCas9 in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   S. pyogenes cas9 with humanized codon bias
   

   Use

   Tags

   myc

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE hSPC-dsRed Express-W

   Plasmid

   #36449

   Depositor

   Darrell Kotton

   Article

   Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

   Inserts

   human SP-C promoter
   dsRed Express
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   N/A and human SP-C promoter

   Available since

   May 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sar1b-H79G-mOrange2

   Plasmid

   #166900

   Purpose

   mammalian expression of Sar1b-H79G tagged with mOrange2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   Sar1b
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   H79G

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB16 (pmyo-3::QuasAr)

   Plasmid

   #130272

   Purpose

   Expresses the voltage sensor QuasAr in BWMs of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   QuasAr
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRB17

   Plasmid

   #52527

   Purpose

   serves as template for PCR-mediated fusion of U6-promoter with sgRNA

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Drosophila U6C promoter
   

   Use

   CRISPR; Blunt cloning kit

   Tags

   T7-promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1-pDUXCca

   Plasmid

   #198236

   Purpose

   Lentivector with tet-inducible porcine DUXC (codon altered)

   Depositor

   Stephen Tapscott

   Article

   Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

   Insert

   porcine DUXC (codon altered)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon altered

   Promoter

   TRE

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPbU6_hdhfr/yfcu_Cas9

   Plasmid

   #216423

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus-GalT

   Plasmid

   #11931

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-ING1b

    Plasmid

    #79052

    Purpose

    expression of p33ING1b in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

    Insert

    ING1 (p33 ING1Ab isoform) (ING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRvACT-mCherry

    Plasmid

    #205002

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUSE-DEST_R1R4

    Plasmid

    #97186

    Purpose

    For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

    Depositor

    Kenneth Scott

    Article

    Lu et al Cancer Res. 2017 May 16. pii: canres.274

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQUAST-shibire (ts1)

    Plasmid

    #24362

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-ReaChR-citrine

    Plasmid

    #169045

    Purpose

    Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-citrine
    

    Use

    Gateway entry cloning vector

    Tags

    citrine

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS416-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158148

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first position of th…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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