We narrowed to 1,548 results for: SIR
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Plasmid#115896PurposeUsed for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertKNL1-M3 (KNL1 Human)
TagsEYFPExpressionMammalianMutationFragment of KNL1 containing three KNL1 repeat seq…Available SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta2-29
Plasmid#124923PurposeFor expression of a.a.2-29 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
TagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-RPB1-His CRres K619,627,642R si2,4R
Plasmid#139409PurposeDox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-RPB1-His K751,758,767R CRres si2,4R
Plasmid#139408PurposeDox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-RPB1-His K710,719R CRres si2,4R
Plasmid#139407PurposeDox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP∆678-681-shTACC3
Plasmid#69115PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of 678-681aa region. Silent mutations i…PromoterCMVAvailable SinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1, V55D
Plasmid#180651PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-Eps8L2_1-496
Plasmid#196516PurposeExpression of GST-Eps8L2_1-496DepositorInsertEps8L2_1-496 (EPS8L2 Human)
TagsGSTExpressionBacterialMutationSilent mutations for siRNA resistancePromoterlac promoterAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM1-COMP5AP-AviTag-9xHis
Plasmid#157060PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut1-2
Plasmid#124918PurposeFor stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTR-471 IRF4-tNGFR
Plasmid#186061PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-msMMS22L
Plasmid#221014Purposemammalian expression vector of eGFP tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
TagseGFPExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable SinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-POLE1
Plasmid#215140PurposeExpresses mCherry-POLE1 in mammalian cells from a retroviral vector.DepositorInsertPOLE1 (POLE Human)
UseRetroviralTagsmCherry_FWRExpressionMammalianMutationSilent mutations in amino acid 80-86 and 211-217 …PromoterCMVAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-53BP1
Plasmid#52507Purposemammalian expression vector of 53BP1DepositorInsert53BP1 (TP53BP1 Human)
TagsFlagExpressionMammalianMutationV128A and siRNA resistant sequence: ACGCGCTGACGAC…PromoterCMV/TOAvailable SinceMarch 26, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF3386
Plasmid#144862PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 deltaCRIM
Plasmid#124921PurposeFor expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)DepositorInsertMAPKAP1 (MAPKAP1 Human)
TagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 deltaPH
Plasmid#124922PurposeFor expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)DepositorInsertMAPKAP1 (MAPKAP1 Human)
TagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c-AtMIR173
Plasmid#137885PurposePlant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.DepositorInsertsAtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos
ExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Available SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG membrane-bound lipin-1 (TMD)
Plasmid#120277PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorInsertLipin-1 (Lpin1 Mouse)
TagsFLAG and in-frame fusion with the TMD Golgi-local…ExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only