We narrowed to 29,051 results for: ple

1. pFA6a-kanMX6-3nmt1-mMaple3

   Plasmid

   #105167

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   kanMX6-3nmt1-mMaple3
   

   Use

   Tags

   3nmt1-mMaple3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   3nmt1

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti.PGK.blast-Flag-MFF-S155,172E

   Plasmid

   #74443

   Purpose

   Lentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 155 Glu, Ser 172 Glu (Numbering based on MFF …

   Promoter

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLenti4/V5-DEST-P85(G376R)

   Plasmid

   #40220

   Depositor

   Lynda Chin

   Insert

   P85(G376R) (PIK3R1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Glycine 376 to Arginine

   Promoter

   CMV

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pLenti CMV_mIFP IRES EGFP

   Plasmid

   #72443

   Purpose

   Expressing mIFP and EGFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   mIFP T2A HO1 IRES EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pRSET-triple mutation-pa-GCaMP6f

   Plasmid

   #67570

   Purpose

   bacterial expression of a photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   pa-GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   V115H, L64F, T65S

   Promoter

   T7

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLenti EPICode screen backbone

   Plasmid

   #177198

   Purpose

   lentiviral vector backbone for both barcodes and KTR

   Depositor

   Markus Covert

   Article

   Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-Ly6G-miR30

   Plasmid

   #163358

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   Ly-6G (Ly6g Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-LmoCas6-KRAB-2a-BlastR

   Plasmid

   #126492

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-KRAB-2a-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-X1-Neo-DDRGK1-K267R-HA

   Plasmid

   #139848

   Purpose

   Lentiviral expression of human DDRGK1-K267R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   K267R

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TRE-PtenC124S-Blast

    Plasmid

    #135675

    Purpose

    Dox-inducible expression (TRE promoter) of C124S mutant Pten cDNA, Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti4/V5-DEST-P85(W583del)

    Plasmid

    #40229

    Depositor

    Lynda Chin

    Insert

    P85(W583del) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 583

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-HF1-PGK-Puro

    Plasmid

    #110857

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV Hygro DHFR.cHSF1

    Plasmid

    #134738

    Purpose

    Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202

    Promoter

    CMV

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

    Plasmid

    #160641

    Purpose

    Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-TCRb-FKBP-Blasticidin

    Plasmid

    #120723

    Purpose

    Expresses ER-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    T cell receptor β chain
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-X1-Neo-HA-UFL1-RAMP4

    Plasmid

    #139863

    Purpose

    Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFL1-RAMP4 (UFL1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    recombinant fusion of UFL1 (full length) with RAM…

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6/V5-DEST-p110 CUX1

    Plasmid

    #100816

    Purpose

    Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

    Depositor

    Alain Nepveu

    Article

    Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1

    Insert

    Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)
    

    Use

    Lentiviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    contains amino acids 747-1505

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-X1-Neo-DDRGK1-K-less-HA

    Plasmid

    #139849

    Purpose

    Lentiviral expression of human DDRGK1-K-less-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid

    #160564

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Edit (En-1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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