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We narrowed to 111,770 results for: promoter

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Showing: 1501 - 1520 of 111770 results

  2. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    60229-plasmid-map-sequence-id-419029

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  3. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    187242-plasmid-map-sequence-id-374822

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  4. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    47868-plasmid-map-sequence-id-68833

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  7. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    10 Articles
    47024-plasmid-map-sequence-id-67042

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  13. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    60231-plasmid-map-sequence-id-417804

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  14. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Publication
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    111905-plasmid-map-sequence-id-512037

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  16. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid
    #58466
    Purpose
    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do
    Inserts
    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    58466-plasmid-map-sequence-id-410899

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  19. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  20. p406ADH1

    Plasmid
    #15974
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    15974-plasmid-map-sequence-id-8459

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Showing: 1501 - 1520 of 111770 results