-
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-sTagRFP-TUBA1B
Plasmid#207769PurposeDonor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC118-FLIP-FlpE-Puro
Plasmid#84539Purposea mock vector for generating FLIP-FlpE targeting vectorsDepositorInsertFLIP-FlpE - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti SpBsmBI sgRNA Puro
Plasmid#62207PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-OVOL1-T2A-PuroR
Plasmid#162347PurposeLentiviral expression of OVOL1 under the control of the TetON promoterDepositorInsertOVOL1 (OVOL1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-PFDN2
Plasmid#202664PurposeTet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassetteDepositorInsertprefoldin subunit 2 (PFDN2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-WNK3
Plasmid#222674Purposeendogenously tag WNK3 with N-terminal mNeonDepositorInsertWNK3 (WNK3 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2-Myc
Plasmid#161583Purposeexpression of full-length mouse Lrrk2 with C-terminal Myc tagDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
OCT4-eGFP-PGK-Puro
Plasmid#31937PurposeOct4 targeting donor vectorDepositorInsertOCT4 (POU5F1 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA
Plasmid#218527PurposesgRNA targeting human EIF2AK2/PKRDepositorInsertEIF2AK2 (EIF2AK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_06-mEGFP
Plasmid#194693PurposeCMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transductionDepositorInsertCaprola_06-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPRv2-PURO-LYSET-gRNA1
Plasmid#210489PurposeCRISPR pooled KO of human LYSETDepositorInsertLYSET-gRNA1 (LYSET Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPRv2-PURO-LYSET-gRNA2
Plasmid#210490PurposeCRISPR pooled KO of human LYSETDepositorInsertLYSET-gRNA2 (LYSET Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro
Plasmid#167911PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…PromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-2A-TurboGFP-PEST-PuroR
Plasmid#80956PurposeCRISPaint gene taggingDepositorInsertTurboGFP-PEST
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-ACTB
Plasmid#227320PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)DepositorInsertACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_on-mEGFP
Plasmid#194694PurposeCMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertCaprola_on-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_off-mEGFP
Plasmid#194695PurposeCMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertCaprola_off-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only