161,398 results

1. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

   Viral Prep

   #26971-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   EYFP

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

2. TFORF0675

   Plasmid

   #141659

   Purpose

   Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM16 (PRDM16 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-GATA4

   Plasmid

   #192896

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Codon-optimized

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Green monkey (Chlorocebus sabaeus) sgRNA library

   Pooled Library

   #178284

   Purpose

   Genome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAs

   Depositor

   John Doench, David Root

   Article

   Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

   Expression

   Mammalian

   Species

   Chlorocebus sabaeus (african green monkey)

   Use

   CRISPR and Lentiviral

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

   Plasmid

   #210725

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   C2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Yarrowia lipolytica Golden Gate tool kit

   Plasmid Kit

   #1000000167

   Purpose

   Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

   Depositor

   Tristan Rossignol

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. scAAV-sgRNA-GFP

   Plasmid

   #177935

   Purpose

   Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AdenoBuilder toolkit

    Plasmid Kit

    #1000000176

    Purpose

    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.

    Depositor

    Fred Bunz

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only