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Showing: 3801 - 3850 of 17876 results

  7. LHP398 - Ub-K48R/K63R

    Plasmid
    #32167
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K48R and K63R
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pGAL-flp(H305L)-NAT

    Plasmid
    #160380
    Purpose
    overexpression of Flp recombinase step-arrest mutant in yeast
    Depositor
    John McCusker
    Insert
    FLP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160380-plasmid-map-sequence-id-314503

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  14. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Use
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113397-plasmid-map-sequence-id-217374

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  15. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  16. LHP938 - Gal1-HA-Ub

    Plasmid
    #32175
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  22. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. LHP599 - pACT2-Ub

    Plasmid
    #32170
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Gal4 AD and HA
    Expression
    Yeast
    Mutation
    Promoter
    pADH1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. pTH340-SUP45(1-242)-GalBD

    Plasmid
    #29740
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-242 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29740-plasmid-map-sequence-id-13824

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  33. Sc RFC[2+3+4]-pET

    Plasmid
    #239203
    Purpose
    Overexpress Sc RFC2, RFC3, & RFC4 in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC2 (RFC2 Budding Yeast)
    RFC3 (RFC3 Budding Yeast)
    RFC4 (RFC4 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239203-plasmid-map-sequence-id-474660

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  34. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  35. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  36. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  37. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  38. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  39. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  40. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  41. pAIP4_3V46

    Plasmid
    #234165
    Purpose
    Heterologous protein expression of cell division control protein 73 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3V46 (CDC73 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234165-plasmid-map-sequence-id-464131

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  42. pRS313_MSN5-LY00045

    Plasmid
    #232904
    Purpose
    Plasmid carrying MSN5 from LY00045 (S288C) with its native promoter and terminator.
    Depositor
    Jolanda Van Leeuwen
    Insert
    MSN5 (MSN5 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232904-plasmid-map-sequence-id-462086

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  43. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

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  44. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

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  45. pHflu3

    Plasmid
    #203881
    Purpose
    pHI1 with insertion of kanamycin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    aminoglycoside O-phosphotransferase APH(3')-IIa
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203881-plasmid-map-sequence-id-403829

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  46. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  47. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

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  48. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166109-plasmid-map-sequence-id-330317

    Enlarge

  49. LHP809 - Ub-K63A-CYC1

    Plasmid
    #32166
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K63A
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  50. LHP327 - Ub-F45A/G76A

    Plasmid
    #32165
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F45A and G76A
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 3801 - 3850 of 17876 results