We narrowed to 2,594 results for: gem
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Plasmid#154951PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…PromoterSynapsinAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet-Blast
Plasmid#169221PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet-P2A-Blast
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean
Plasmid#154948PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterCaMKIIa(0.4)Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet-Puro
Plasmid#169220PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mNeongreen-Puro
Plasmid#169228PurposeTargeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7-W
Plasmid#159292PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoterDepositorInsertKAT7 (KAT7 Human)
UseLentiviralExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10)Promoterhuman EF1a promoterAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianPromoterhuman synapsinAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mNeongreen-Blast
Plasmid#169229PurposeTargeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen-P2A-Blast
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-VenusYFP-3AT
Plasmid#71269PurposeEntry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTags4xGly linker and T3A pea Pisum sativum ribulose-1…Available SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE
Plasmid#118274PurposeExpresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoterDepositorInsertsbPAC(WT)
SthK
UseAAVTagsHis tag, P2A, mCherry, and myc tagExpressionMammalianPromoterCamK IIAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HLA-c-myc-optiT1R3 ECD-MHC
Plasmid#113961Purposemammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonicalDepositorInsertT1R3 (TAS1R3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 21-563 fused to a transmembrane tetherPromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Hygro
Plasmid#169225PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Hygro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV GST mTagBFP
Plasmid#206259PurposeENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.DepositorInsertGTS mTagBFP
UseMultimate/gateway entr 4TagsBFGT1 (Golgi localisation peptide)ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV H2B iRFP
Plasmid#206253PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertH2B iRFP713
UseRecombinant baculovirus production, multimate/gat…TagsiRFP713ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 CMV mKO2-hCdt1
Plasmid#206282PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMVDepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Puro
Plasmid#169224PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_CA_Citrine
Plasmid#98218PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…Promoterhuman synapsinAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_iChloC_CA_Citrine
Plasmid#98220PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterhuman synapsinAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only