We narrowed to 6,096 results for: pcDNA 3.1

1. Human NCKX4-AS-long (pcDNA3.1+)

   Plasmid

   #75211

   Purpose

   Mammalian expression of SLC24A4

   Depositor

   Jonathan Lytton

   Article

   Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

   Insert

   SLC24A4 (SLC24A4 Human)
   

   Expression

   Mammalian

   Mutation

   alternatively spliced exon present

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 human cofilin S3A

   Plasmid

   #50854

   Purpose

   expresses constituvely active cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Expression

   Mammalian

   Mutation

   constitutively active, S3A

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCDNA3.1(+)-myc-SNAP-GP135

   Plasmid

   #50376

   Purpose

   Express myc and SNAP-tagged GP135 in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   SNAP
   

   Expression

   Mammalian

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)_Strep-Lrrk2-Myc

   Plasmid

   #161582

   Purpose

   expression of full-length mouse Lrrk2 with C-terminal Myc tag

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LARG mtq sspB in pcDNA3.1

   Plasmid

   #90460

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mTq
   sspB
   

   Expression

   Mammalian

   Mutation

   R73Q and deletion a.a. (234-239)

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Ost4-GFP-MTn

   Plasmid

   #154351

   Purpose

   Mammalian expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

   Plasmid

   #98041

   Purpose

   Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Insert

   Sa-dCas9-NLS-3xFLAG
   

   Use

   CRISPR

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   D10A + H557A

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 6A CARD8 CARD-FLAG WT

   Plasmid

   #164020

   Purpose

   Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-V5H6-mPCSK3 D153A

   Plasmid

   #122681

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 153 to Alanine

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-Mito-AcGFP-MTn

    Plasmid

    #154350

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    AcGFP

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid

    #118407

    Purpose

    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-NES
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CMV-mCherry-hPMCA2x/b

    Plasmid

    #111571

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-OTUB1 T134R-pcDNA3.1

    Plasmid

    #118211

    Purpose

    Expresses FLAG-OTUB1 T134R mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 134 to arginine

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ERBB4 (kinase dead)

    Plasmid

    #29533

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid

    #85221

    Purpose

    Role of Rac selective GEF domain from Tiam1 in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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