We narrowed to 16,609 results for: puromycin

1. pYJ36-PB-CRISPR-NKX3.1-E1-gRNA1

   Plasmid

   #131079

   Purpose

   NKX3-1 knock out

   Depositor

   Xiaojun Lian

   Insert

   NKX3.1 Exon1 gRNA1 (NKX3-1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYJ37-PB-CRISPR-NKX3.1-E1-gRNA2

   Plasmid

   #131080

   Purpose

   NKX3-1 knock out

   Depositor

   Xiaojun Lian

   Insert

   NKX3.1 Exon1 gRNA2 (NKX3-1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYJ38-PB-CRISPR-NKX3.1-E2-gRNA1

   Plasmid

   #131081

   Purpose

   NKX3-1 knock out

   Depositor

   Xiaojun Lian

   Insert

   NKX3.1 Exon2 gRNA1 (NKX3-1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNpF-deleted

   Plasmid

   #245372

   Purpose

   Negative control, luciferase cassette deleted

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-T-PAF-VLDLR_ecto_short isoform

   Plasmid

   #215395

   Purpose

   Expression of the ectodomain(1-797) of the short isoform of the Very Low-Density Lipoprotein Receptor (VLDLR, UniProtKB; P98155-2) fused to a C-tag (EPEA) in th C- terminal for purification.

   Depositor

   Franz-Ulrich Hartl

   Insert

   VLDLR (VLDLR Human)
   

   Tags

   C-tag (EPEA)

   Expression

   Mammalian

   Mutation

   p.Ala798_Ala873del

   Promoter

   tetO

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2-ACTB-C4

   Plasmid

   #229849

   Purpose

   Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. scFv-SmBiT

   Plasmid

   #192541

   Purpose

   Expresses sender cell components of LOTIIS

   Depositor

   Jennifer Prescher

   Article

   Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

   Insert

   scFv - SmBiT86
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX ALIX-GFP S718/721/E

   Plasmid

   #138584

   Purpose

   Expresses fluorescent ALIX S718/721E in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Mutation

   changed serines 718 and 721 to glutamic acids

   Promoter

   TREtight

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3GEPIR-CALM1shRNA1

   Plasmid

   #220567

   Purpose

   To inducibly knockdown CALM1 expression

   Depositor

   Sean Lee

   Insert

   CALM1 shRNA1 (CALM1 Human)
   

   Use

   Lentiviral

   Promoter

   TRE3G (TetOP) promoter

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LT3GEPIR-CALM1shRNA2

    Plasmid

    #220568

    Purpose

    To inducibly knockdown CALM1 expression

    Depositor

    Sean Lee

    Insert

    CALM1 shRNA2 (CALM1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMta2-EosHalo-TAFiP

    Plasmid

    #140959

    Purpose

    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    metastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid

    #135260

    Purpose

    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-dsRed
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ELL1 CtoA

    Plasmid

    #127279

    Purpose

    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHNK by Q5 mutagenesis

    Promoter

    EF1a

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)/Puro/FA/LL5BIP-GFP

    Plasmid

    #112831

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pYJA5

    Plasmid

    #217778

    Purpose

    The empty vector for quadruple sgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Puromycin resistance gene, T2A, and TagBFP

    Expression

    Mammalian

    Promoter

    PGK, CMV, U6

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-EGFP-FLAG-PSMD2

    Plasmid

    #161917

    Purpose

    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV Puro DEST (w118-1) SDC3

    Plasmid

    #107501

    Purpose

    Expresses SDC3, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    SDC3 (codon optimized) (SDC3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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