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Showing: 1561 - 1580 of 1801 results

  6. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

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  7. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40131-plasmid-map-sequence-id-51019

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  10. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  12. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  16. pRSF-G1(6/7FTrp)RS

    Plasmid
    #207621
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    207621-plasmid-map-sequence-id-410952

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  17. pRSF-G1(4/5FTrp)RS

    Plasmid
    #207620
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    207620-plasmid-map-sequence-id-435603

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  19. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  20. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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Showing: 1561 - 1580 of 1801 results