We narrowed to 24,337 results for: SPR

1. pET28b_TypeIC_acrIC4_invivo

   Plasmid

   #196403

   Purpose

   expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Insert

   anti-CRISPR IC4 protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2-AcrIIC3

   Plasmid

   #174036

   Purpose

   In vitro transcription of anti-CRISPR AcrIIC3 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   AcrIIC3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-AcrIIC1

   Plasmid

   #174035

   Purpose

   In vitro transcription of anti-CRISPR AcrIIC1 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   AcrIIC1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JME5000

   Plasmid

   #129661

   Purpose

   EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEL086

   Plasmid

   #137894

   Purpose

   iSTU-CRISPR/Cas9 with RcCat intron, RZ structure backbone

   Depositor

   Yong Zhang

   Article

   Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEL077

   Plasmid

   #137886

   Purpose

   iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone

   Depositor

   Yong Zhang

   Article

   Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEL079

   Plasmid

   #137888

   Purpose

   iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone

   Depositor

   Yong Zhang

   Article

   Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEL078

   Plasmid

   #137887

   Purpose

   iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone

   Depositor

   Yong Zhang

   Article

   Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYZ292

   Plasmid

   #102686

   Purpose

   S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KRAB-dSpCas9

    Plasmid

    #205248

    Purpose

    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge