Skip to main content
Addgene
Showing: 211 - 240 of 648 results

  1. pBFC1207

    Plasmid
    #186694
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186694-plasmid-map-sequence-id-371385

    Enlarge

  6. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

    Enlarge

  7. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

    Enlarge

  9. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

    Enlarge

  10. pET-PesaRlux

    Plasmid
    #47802
    Purpose
    PesaR promoter, luxCDABE, and terminator in pET17b (AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-luxCDABE-term
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PesaR
    Available since
    Sept. 6, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pHBSTC146

    Plasmid
    #124357
    Purpose
    Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein (C1G) and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    C1G
    Promoter
    STC
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124357-plasmid-map-sequence-id-241737

    Enlarge

  17. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

    Enlarge

  18. pET-PesaRlux

    Plasmid
    #47802
    Purpose
    PesaR promoter, luxCDABE, and terminator in pET17b (AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-luxCDABE-term
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PesaR
    Available since
    Sept. 6, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pBHSTC97

    Plasmid
    #124352
    Purpose
    Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 mini-intein
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124352-plasmid-map-sequence-id-242354

    Enlarge

  29. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

    Enlarge

Showing: 211 - 240 of 648 results