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Showing: 351 - 400 of 27714 results

  1. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  2. p2B-57

    Plasmid
    #177927
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET3 (contains three rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177927-plasmid-map-sequence-id-349993

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  3. pmSod1-Q42L;Q123A-N’His

    Plasmid
    #117710
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L; Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117710-plasmid-map-sequence-id-227524

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  4. pGCC4

    Plasmid
    #37058
    Depositor
    Hank Seifert
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    37058-plasmid-map-sequence-id-352924

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  5. pGCC5

    Plasmid
    #37059
    Depositor
    Hank Seifert
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-Syn-SomaGCaMP7

    Plasmid
    #158759
    Purpose
    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158759-plasmid-map-sequence-id-310665

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  7. pAAV-Syn-SomaGCaMP6f2

    Plasmid
    #158756
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158756-plasmid-map-sequence-id-310660

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  8. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

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  9. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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  10. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid
    #158758
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    nullCoChR-GCaMP6f-Kv2.1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158758-plasmid-map-sequence-id-310664

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  11. pCMV-HA (New MCS)

    Plasmid
    #32530
    Depositor
    Christopher A Walsh
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32530-plasmid-map-sequence-id-351565

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  12. Flag-WASP

    Plasmid
    #47432
    Depositor
    Peter McPherson
    Insert
    WASP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. Endophilin II Full

    Plasmid
    #47409
    Depositor
    Peter McPherson
    Insert
    Endophilin II
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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  15. FNpCDNA3

    Plasmid
    #45346
    Depositor
    Robert Oshima
    Type
    Empty backbone
    Use
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45346-plasmid-map-sequence-id-63229

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  16. pAK_Tol2_TRE_Hygro

    Plasmid
    #130260
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    130260-plasmid-map-sequence-id-310817

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  17. pAK_Tol2_TRE_Puro

    Plasmid
    #130259
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130259-plasmid-map-sequence-id-255016

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  18. pAK_Tol2_TRE_Blast

    Plasmid
    #130261
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130261-plasmid-map-sequence-id-255018

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  19. pAK_Tol2_TRE_Zeo

    Plasmid
    #130263
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130263-plasmid-map-sequence-id-255025

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  20. pTpPBR11_fcpNAT

    Plasmid
    #154035
    Purpose
    Diatom episome backbone for T. pseudonana
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154035-plasmid-map-sequence-id-307158

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  21. pPtPBR11_fcpShble_GWB

    Plasmid
    #154031
    Purpose
    Gateway cloning into P. tricornutum episomal vector
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154031-plasmid-map-sequence-id-307050

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  22. Endophilin III Full

    Plasmid
    #47410
    Depositor
    Peter McPherson
    Insert
    Endophilin III
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pTpPBR11_accNAT

    Plasmid
    #154034
    Purpose
    Diatom episome backbone for T. pseudonana or C. crytpica
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154034-plasmid-map-sequence-id-307157

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  24. pTpPBR11_fcpNAT_GWB

    Plasmid
    #154032
    Purpose
    Gateway cloning into T. pseudonana episomal vector
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154032-plasmid-map-sequence-id-307051

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  25. pAK_Tol2_TRE_Neo

    Plasmid
    #130262
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130262-plasmid-map-sequence-id-310816

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  26. pTpPBR11_accNAT_GWB

    Plasmid
    #154033
    Purpose
    Gateway cloning into T. pseudonana or C. cryptica episomal vector
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154033-plasmid-map-sequence-id-307556

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  27. pUX19

    Plasmid
    #36328
    Depositor
    Gary Roberts
    Type
    Empty backbone
    Use
    Suicide vector for r. rubrum
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    reduce strength lacZ
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  28. pKAM-GFP

    Plasmid
    #101865
    Purpose
    Lentiviral empty backbone, EGFP marker
    Depositor
    Archibald Perkins
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    May 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    101865-plasmid-map-sequence-id-211114

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  29. pFRT-Tet 129

    Plasmid
    #33359
    Depositor
    Doug Mortlock
    Insert
    TetR cassette
    Use
    Mouse Targeting
    Tags
    FRT site
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  30. pRY003

    Plasmid
    #81043
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HO
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    endogenous
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. MIG-Luciferase-IRES-GFP

    Plasmid
    #75021
    Purpose
    Encodes the expression of Luciferase and GFP
    Depositor
    Saradhi Mallampati
    Insert
    Luciferase
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    75021-plasmid-map-sequence-id-337469

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  32. MSCV-Luciferase-IRES-mCerulean

    Plasmid
    #75022
    Purpose
    Encodes the expression of Luciferase and mCerulean
    Depositor
    Saradhi Mallampati
    Insert
    Luciferase
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. GK Rab3A p201

    Plasmid
    #44696
    Depositor
    Geri Kreitzer
    Insert
    Rab3A (RAB3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. GFP-Intersectin Full Length

    Plasmid
    #47407
    Depositor
    Peter McPherson
    Insert
    Intersectin
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

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  36. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177925-plasmid-map-sequence-id-349995

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  37. phSOD1-I113T-N’His

    Plasmid
    #117703
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    I113T
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117703-plasmid-map-sequence-id-227513

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  38. phSOD1-E100G-N’His

    Plasmid
    #117706
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    E100G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117706-plasmid-map-sequence-id-227528

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  40. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  41. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

    Enlarge

  42. GK Rab6A p201

    Plasmid
    #44700
    Depositor
    Geri Kreitzer
    Insert
    Rab6A (RAB6A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pmTCOF1

    Plasmid
    #79278
    Purpose
    Bacterial expression of TCOF1
    Depositor
    Rita Shiang
    Insert
    TCOF1 (Tcof1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. GK Rab5A p201

    Plasmid
    #44699
    Depositor
    Geri Kreitzer
    Insert
    Rab5A (RAB5A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  45. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    134843-plasmid-map-sequence-id-266705

    Enlarge

  46. pNPTS138 Cm

    Plasmid
    #41891
    Depositor
    Howard Steinman
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    41891-plasmid-map-sequence-id-365602

    Enlarge

  47. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

    Enlarge

  48. pAK_DR30_CasRx_Puro

    Plasmid
    #134845
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    134845-plasmid-map-sequence-id-266706

    Enlarge

  49. pSR47S Cm

    Plasmid
    #41892
    Depositor
    Howard Steinman
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

    Enlarge

Showing: 351 - 400 of 27714 results