We narrowed to 11,841 results for: NSI
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Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mix.2-lux
Plasmid#20893DepositorInsert-290 Mix.2 promoter luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacIAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)-c-term-cpGFP-ERexp
Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVd2Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Actinin-sstFRET-GR
Plasmid#83416PurposeFRET-based actinin tension sensorDepositorInsertActinin-sstFRET-GR
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
negActinin-sstFRET-GR
Plasmid#83417PurposeFRET-based actinin tension sensor (negative control)DepositorInsertnegActinin-sstFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G
Plasmid#47646PurposepAC-EsaR with a a272g mutation in esaRDepositorInsertesaR-D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to GlycinePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS-PluxIlux
Plasmid#47641Purposewt luxI promoter in pCS26DepositorInsertPluxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPluxIAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-V220A
Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V
Plasmid#47663PurposepAC-EsaR with a208g mutation in esaRDepositorInsertesaR-I70V
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to ValinePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaRlux
Plasmid#47640Purposewt esaR promoter in pCS26DepositorInsertPesaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Mix.2-lux M-p53BE
Plasmid#20894DepositorInsert-290 Mix.2 M-p53BE promoter luciferase
UseTagsExpressionMammalianMutationpoint mutation of the p53 binding element (p53BE)…PromoterAvailable SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G
Plasmid#47644PurposepAC-EsaR with a208g and a272g mutations in esaRDepositorInsertesaR-I70V/D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine and Aspartic acid…PromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseTagsExpressionMutationPromoterAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only