Addgene

We narrowed to 3,628 results for: WOL

Showing: 141 - 160 of 3628 results

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-L261A

Plasmid

#73671

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pH3U3-zif268 (omega)

Plasmid

#18046

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

zif268 binding site

Use

Tags

Expression

Bacterial

Mutation

The preferred binding site for zif268 is inserted…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-E451A

Plasmid

#73673

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Use

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-Prd

Plasmid

#18038

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

Paired (prd Fly)

Use

Tags

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pB1H2w5-Prd

Plasmid

#18039

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

Paired (prd Fly)

Use

Tags

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pB1H2wL-Prd

Plasmid

#18040

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

Paired (prd Fly)

Use

Tags

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pX-EGFP-g1

Plasmid

#107273

Purpose

eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP sgRNA-1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

mutant Odd Skipped

Use

Tags

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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UBC13-PGEX4T

Plasmid

#25464

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

Ubc13 (UBC13, MMS2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
hOtub1

Plasmid

#26959

Depositor

Cynthia Wolberger

Article

Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

Insert

Otub1 (OTUB1 Human)

Use

Tags

His6 (TEV cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-L261A

Plasmid

#73676

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-eGFP-pA

Plasmid

#107281

Purpose

donor vector backbone with constitutive eGFP

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pS2-FLAG-Sestrin2-L261A

Plasmid

#73678

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

Sesn2

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Flag-ZFP809(1-353)

Plasmid

#67788

Purpose

Mammalian expression of 1-353 amino acids of ZFP809 tagged with Flag

Depositor

Stephen Goff

Article

Wolf et al Nature. 2009 Apr 30;458(7242):1201-4. do

Insert

ZFP809 (Zfp809 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
Ubp8/PET32a

Plasmid

#26965

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Ubp8 (UBP8 Budding Yeast)

Use

Tags

His6 and Trx

Expression

Bacterial

Mutation

TEV site is added t the 5' end and 4 alanine…

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
UbG75C

Plasmid

#37194

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

Ubiquitin G75C mutant (RBX1 Human)

Use

Tags

No tag

Expression

Bacterial

Mutation

Glycine 75 changed to Cystine

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only