We narrowed to 1,324 results for: grna cloning vector

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p102_gRNA_Sa_mClover

Plasmid

#213778

Purpose

Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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p103_gRNA_Sp_mClover

Plasmid

#213779

Purpose

Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-29

Plasmid

#66203

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-1

Plasmid

#66202

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3b

Plasmid

#66198

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3b

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3d

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6c

Plasmid

#66197

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6c

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6b

Plasmid

#66196

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6b

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C6

Plasmid

#160457

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C5

Plasmid

#160456

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C4

Plasmid

#160455

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

CR7T

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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