We narrowed to 6,720 results for: nls

1. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

   Plasmid

   #217478

   Purpose

   Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

   Depositor

   Peng Zou

   Article

   Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

   Insert

   Nier1s
   

   Use

   Tags

   3xNLS, P2A-EGFP-3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

   Plasmid

   #217479

   Purpose

   Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

   Depositor

   Peng Zou

   Article

   Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

   Insert

   Nier1b
   

   Use

   Tags

   3xNLS, P2A-EGFP-3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

   Plasmid

   #191365

   Purpose

   Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dPbaCas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

   Plasmid

   #191364

   Purpose

   Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dPba5Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

   Plasmid

   #191363

   Purpose

   Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dPba4Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

   Plasmid

   #191366

   Purpose

   Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dHgm6Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

   Plasmid

   #191362

   Purpose

   Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dBba2Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

   Plasmid

   #191361

   Purpose

   Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dPba3Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

   Plasmid

   #183399

   Purpose

   Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

    Plasmid

    #121836

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid

    #121837

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

    Plasmid

    #121838

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid

    #121840

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69228

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69229

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69230

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

    Plasmid

    #176271

    Purpose

    Expression of CasMINI-V3.1 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI-V3.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

    Plasmid

    #226514

    Purpose

    Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132397

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1058A

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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