Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,562 results for: nls

Clear keywords
Showing: 141 - 160 of 6562 results

  2. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    62202-plasmid-map-sequence-id-288285

    Enlarge

  11. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has service
    AAV1 and AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    51085-plasmid-map-sequence-id-148706

    Enlarge

  14. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    138147-plasmid-map-sequence-id-349848

    Enlarge

  15. SQT1665

    Plasmid
    #78744
    Purpose
    Human expression plasmid for LbCpf1-NLS-3xHA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    78744-plasmid-map-sequence-id-147455

    Enlarge

  18. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    131300-plasmid-map-sequence-id-254651

    Enlarge

  19. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has service
    AAV1 and AAV Retrograde
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    51084-plasmid-map-sequence-id-172966

    Enlarge

Showing: 141 - 160 of 6562 results