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PGL4-4X TE Down (GQs) Renilla

Plasmid

#70039

Purpose

Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

4x TE Down (G-quadruplexes) in 5'UTR

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Mutation

4 repeats of TE down motif (G-quadruplexes) in 5&…

Promoter

SV40

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pIS1-Eef25UTR-TOPmut-renilla

Plasmid

#38236

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

The first nucleotides of the 5' UTR were cha…

Promoter

Endogenous Eef2

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pLV x EF1a_EKAREN5-ires-blast

Plasmid

#167822

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Expression

Mammalian

Promoter

RNA PolIII 128 subunit (RpIII128)

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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CA-0030 CMVTO-Renin-ECL

Plasmid

#231189

Purpose

Membrane-bound human renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin-ECL (REN Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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Incoherent feedforward (IFF) plasmid

Plasmid

#169748

Purpose

Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-MYB-shRNA

Plasmid

#25790

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human MYB (MYB Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
Beta-2-adrenergic receptor-mCerulean

Plasmid

#66957

Purpose

Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mCerulean (ADRB2 Human, Aequorea victoria)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-BPTF-sh5

Plasmid

#83045

Purpose

MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)

Use

Retroviral

Available Since

Oct. 24, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSuperGFP-cdc42-prenyl siRNA

Plasmid

#22715

Depositor

Alaa El-Husseini

Insert

cdc42 siRNA (Rac1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-CDK4-shRNA

Plasmid

#25788

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK4 (CDK4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
hADAM10 siRNA pSIREN-Retro-Q

Plasmid

#19140

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 siRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
ZE33 Prickle-1-GFPnopren-pCS2+

Plasmid

#17074

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Mutation

Pk1GFPnopren deletes the C-terminal six amino aci…

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

Plasmid

#167819

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

Plasmid

#167820

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

Plasmid

#167827

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-BPTF-sh3

Plasmid

#73667

Purpose

pSIREN-RetroQ vector containing shRNA sequence to BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #3 to BPTF (BPTF, Bptf Human, Mouse)

Use

Retroviral

Available Since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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