We narrowed to 9,138 results for: Ott

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W)

Plasmid

#251552

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY_V106W

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY)

Plasmid

#251544

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAPf-Sec61b

Plasmid

#247295

Purpose

Mammalian expression of the SNAPf-tagged ER reporter SEC61b; to label ER membranes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SNAPf-Sec61b (SEC61B Human)

Tags

SnapTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CrPV-IRES-cytERM-SUNTAG-MS2

Plasmid

#247299

Purpose

Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

PB-CrPV-IRES-cytERM-SUNTAG-MS2

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-cyto

Plasmid

#229694

Purpose

Transient expression of EGFP AP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-cyto

Plasmid

#229695

Purpose

Transient expression of FP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMC6-spacer

Plasmid

#176178

Purpose

yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

41 bp non-coding DNA spacer from pYTK048

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168168

Purpose

Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168171

Purpose

Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

Plasmid

#130684

Purpose

Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D453_F489del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

Plasmid

#130683

Purpose

Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_E452del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTIM

Plasmid

#81198

Purpose

pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

Depositor

Andrés Vázquez-Torres

Article

Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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