We narrowed to 4,158 results for: SPL

Showing: 1581 - 1600 of 4158 results

Results per page:

Sort results by:

mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

Human ferritin

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All VLIM to F in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_hE2F7mg

Plasmid

#73073

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_(GLuc)_INT[P4-P6(3xPP7)]

Plasmid

#68432

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt2B2

Plasmid

#35907

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV7.1_3xFLAG-TAOK2

Plasmid

#172992

Purpose

constitutive expression of FLAG-tagged TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK2 (TAOK2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-MHL: nsp10_SARS-CoV-2

Plasmid

#167851

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

Plasmid

#140226

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

Plasmid

#133326

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of CTD in full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

Plasmid

#133324

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of NTD in full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 AK3 Hyg

Plasmid

#37539

Depositor

David Sidransky

Article

Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

Insert

AK3 (AK3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
SFG.CNa12_opt.IRES.eGFP

Plasmid

#22490

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 12 (PPP3CA Human)

Use

Retroviral

Mutation

T351E; L354A

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Article

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase C2029A

Plasmid

#59822

Purpose

Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN-HA-CYLD

Plasmid

#27556

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Available Since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only