We narrowed to 5,563 results for: cycs
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Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKC beta II CAT
Plasmid#16384DepositorInsertPKC beta II (Prkcb Mouse)
TagsHAExpressionMammalianMutationCAT: catalytic domain, aa 329-673Available SinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidPromoterCYC1Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA3174
Plasmid#46880PurposeLentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domainDepositorTypeEmpty backboneUseLentiviralTagsFFKBP12 E31G, R71G, K105E and mycPromoterTRE-TightAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyExpressionBacterialPromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_TIAL1
Plasmid#106090Purposegene expression of FlagHA-TIAL1DepositorAvailable SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zfp521 full length
Plasmid#104189PurposeExpression of full length Zfp521 in mammalian cellsDepositorAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dSaCas9-KRAB-gRNA-TRE-blast
Plasmid#201151PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsSadCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAMutationgRNA sequence: ATCAGTGATAGAGAACGTATGAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-APC11
Plasmid#19900DepositorAvailable SinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec31-yoHalo
Plasmid#115423PurposeExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec31-yoHalo
TagsyoHaloTagExpressionYeastPromoterEndogenousAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKI1
Plasmid#199118PurposeExpresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labellingDepositorInsertH6-SNAP-RapA
Tags6xHis and SNAP-tagExpressionBacterialAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TfRΔEctoR1-eGFP A206K
Plasmid#113554PurposeTransmembrane model receptor to probe receptor internalization via clathrin mediated pathwayDepositorInsertTfRΔEctoR1-eGFP A206K
ExpressionMammalianMutationeGFP A206KAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 WT
Plasmid#27272DepositorAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPMW-attB
Plasmid#61814PurposepUASp plasmid with attB for Drosophila transgenesisDepositorTypeEmpty backboneTags6xMycExpressionInsectPromoterUASpAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_Empty
Plasmid#92015PurposeEmpty backbone, includes IRES-mCherryDepositorTypeEmpty backboneTagsV5ExpressionMammalianAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CasRX_Pac
Plasmid#194803PurposeCasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.DepositorInsertCasRX
Available SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
UbcH10 AU1
Plasmid#8506DepositorAvailable SinceJune 1, 2005AvailabilityAcademic Institutions and Nonprofits only