We narrowed to 25,701 results for: nes
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Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
P447-FBP_19
Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-Blast-Puro
Plasmid#167186PurposeLentiviral expression of sgRNA with blasticidin and puromycin resistance genesDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYihI-C-term K-R
Plasmid#233092PurposeExpression of GST-YihI with C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with C-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationC-term lysines (K) mutated to arginine (R)Available SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorMutationsee comments section belowAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterhUbCAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-Runx1-neo
Plasmid#45815DepositorAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-puro
Plasmid#65232Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N+C-term K-R
Plasmid#233093PurposeExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with N+C-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with N+C-term lysines (K) mutated to arg…Available SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-All K-R
Plasmid#233094PurposeExpression of GST-YihI with all lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with all lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with all lysines (K) mutated to arginine…Available SinceMay 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term S-A
Plasmid#233096PurposeExpression of GST-YihI with N-term serines (S) mutated to alanine (A)DepositorInsertYihI with N-term serines (S) mutated to alanine (A)
TagsGSTExpressionBacterialMutationN-term serines (S) mutated to alanine (A)Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP (empty vector)
Plasmid#172604PurposeEmpty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cellsDepositorTypeEmpty backboneTags2xFLAG-2xSTREPExpressionMammalianAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only