Addgene

We narrowed to 9,529 results for: SUB

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3a(S)-Fc-His

Plasmid

#72138

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only
pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pH2R-P2A-mCherry-N1

Plasmid

#84331

Purpose

Co-expresses (untagged) Histamine-2 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R-P2A-mCherry (HRH2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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YFP-POP5-C1

Plasmid

#134543

Purpose

Expresses YFP-POP5 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

POP5 (POP5 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1518

Plasmid

#205981

Purpose

FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1514

Plasmid

#205977

Purpose

FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8/Cse1 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1515

Plasmid

#205978

Purpose

FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas11/Cse2 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (208-439)

Plasmid

#203433

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1801

Plasmid

#205966

Purpose

JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH proteins and CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1232

Plasmid

#205969

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH proteins and CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP8

Plasmid

#41669

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAMKIIa

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA1

Plasmid

#201626

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA2

Plasmid

#201627

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Neo1.a-Fc-His

Plasmid

#72089

Purpose

Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.a (Neo1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT3035

Plasmid

#122543

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_lmn-1::CemOrange2 (lmn-1 Nematode, Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema5a-Fc-His

Plasmid

#72161

Purpose

Expresses the extracellular region of the Sema5A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema5a (Sema5a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP-mCherry-SEC61B

Plasmid

#207671

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

SEC61B (SEC61B Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Use

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Unc5c.a-Fc-His

Plasmid

#72180

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
px458_2A_GFP_sgRNA_LTN1

Plasmid

#127125

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA LTN1 (LTN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mCherry-FKBP-TIAM1(DHPH)

Plasmid

#176138

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCapV

Plasmid

#183028

Purpose

the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

Depositor

Ute Romling

Article

Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

Insert

patatin-like phospholipase CapV

Use

Tags

No

Expression

Bacterial

Mutation

Promoter

pBAD promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4 CDS A. thaliana PHOT1

Plasmid

#209187

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT3G45780.1 (PHOT1 Mustard Weed)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Susd4(3.7)-EGFP-sv40

Plasmid

#184413

Purpose

AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

Depositor

Marylka Yoe Uusisaari

Article

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Mouse Susd4 gene

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema4d-Fc-His

Plasmid

#72157

Purpose

Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4d (Sema4d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.2/V5-DEST NET1A V5 WT

Plasmid

#69817

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

NET1A (NET1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Rpp40-C1

Plasmid

#134549

Purpose

Expresses YFP-Rpp40 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp40 (RPP40 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3a(L, del13)-AP-His

Plasmid

#72011

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
F-MED28

Plasmid

#15368

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED28 (MED28 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
Ncam1_1.18.1-AP-His

Plasmid

#71962

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ncam1_0.0.0-AP-His

Plasmid

#71960

Purpose

Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_0.0.0 (Ncam1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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