We narrowed to 5,632 results for: chia

1. pTETDuet_D27NDHFR_Q33STS

   Plasmid

   #191289

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D27N DHFR, Q33S TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTETDuet_M42FG121VDHFR_Q33STS

   Plasmid

   #191292

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M42F/G121V DHFR, Q33S TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTETDuet_F31YDHFR_R166QTS

   Plasmid

   #191311

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F31Y DHFR/R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTETDuet_F31YDHFR_WTTS

   Plasmid

   #191310

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F31Y DHFR/WT TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTETDuet_F31YDHFR_Q33STS

   Plasmid

   #191313

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F31Y DHFR/Q33S TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTETDuet_F31YG121VDHFR_Q33STS

   Plasmid

   #191293

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F31Y/G121V DHFR, Q33S TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTETDuet_WTDHFR_R166QTS

   Plasmid

   #191294

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTETDuet_D27NDHFR_R166QTS

   Plasmid

   #191295

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D27N DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTETDuet_M42FDHFR_R166QTS

   Plasmid

   #191296

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M42F DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTETDuet_G121VDHFR_R166QTS

    Plasmid

    #191297

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G121V DHFR, R166Q TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTETDuet_F31YG121VDHFR_R166QTS

    Plasmid

    #191299

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F31Y/G121V DHFR, R166Q TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTETDuet_WTDHFR_R127ATS

    Plasmid

    #191300

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTETDuet_D27NDHFR_R127ATS

    Plasmid

    #191301

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27N DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTETDuet_M42FDHFR_R127ATS

    Plasmid

    #191302

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTETDuet_G121VDHFR_R127ATS

    Plasmid

    #191303

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G121V DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTETDuet_F31YG121VDHFR_R127ATS

    Plasmid

    #191304

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F31Y/G121V DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTETDuet_M42FG121VDHFR_R127ATS

    Plasmid

    #191305

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F/G121V DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTETDuet_D27NDHFR_WTTS

    Plasmid

    #191283

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27N DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTETDuet_M42FDHFR_WTTS

    Plasmid

    #191284

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTETDuet_G121VDHFR_WTTS

    Plasmid

    #191285

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G121V DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTETDuet_M42FG121VDHFR_WTTS

    Plasmid

    #191286

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F/G121V DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTETDuet_F31YG121VDHFR_WTTS

    Plasmid

    #191287

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F31Y/G121V DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTETDuet_WTDHFR_Q33STS

    Plasmid

    #191288

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT DHFR, Q33S TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTETDuet_M42FDHFR_Q33STS

    Plasmid

    #191290

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F DHFR, Q33S TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTETDuet_G121VDHFR_Q33STS

    Plasmid

    #191291

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G121V DHFR, Q33S TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-GST-V5His6_E

    Plasmid

    #146223

    Purpose

    Insect Expression of GST

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    GST
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD33(A-H*HA-N)

    Plasmid

    #187086

    Purpose

    Medium expression of Complex I with subunits N and H -HA tagged

    Depositor

    Steven Vik

    Article

    Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

    Insert

    nuoA-N
    

    Use

    Tags

    HA tags nuoN, nuoH

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSelect-7

    Plasmid

    #190244

    Purpose

    Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSelect-9

    Plasmid

    #190245

    Purpose

    Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. relA+_NS1

    Plasmid

    #100916

    Purpose

    expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    RelA fragment from E.coli
    

    Use

    Cyanobacteria cloning vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBad-HPII TAG206

    Plasmid

    #105846

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 206

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBad-HPII TAG568

    Plasmid

    #105845

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 568

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pECT1B

    Plasmid

    #75447

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pECT1D

    Plasmid

    #75448

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pECT1E

    Plasmid

    #75449

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHL 1571

    Plasmid

    #37760

    Depositor

    Han Lim

    Article

    Hussein et al Nucleic Acids Res. 2012 May 22.

    Inserts

    ptet_ompC_gfpAaV
    pLac_st3 TetR_mCherry
    

    Use

    Synthetic Biology; E. coli

    Tags

    gfp and mCherry

    Expression

    Mutation

    AaV degradation tag added to the 3'end

    Promoter

    pLac and ptet

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pKS100

    Plasmid

    #24630

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    fadD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pCB301-2μ-HDV

    Plasmid

    #196329

    Purpose

    A yeast-Escherichia coli-Agrobacterium shuttle vector

    Depositor

    Zhenghe Li

    Article

    Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDA303

    Plasmid

    #195199

    Purpose

    Overexpressing 6His-TetR in E. coli.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    TetR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MscL(G22S)61-cpGFP-ERexp

    Plasmid

    #195311

    Purpose

    Fluorescent reporter/biosensor for membrane tension

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)61-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PT7lacO-RF1-His6

    Plasmid

    #224372

    Purpose

    Express RF1 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RF1 (prfA E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEHAC1-HA-BirAonly

    Plasmid

    #232584

    Purpose

    Express HA-BirA

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBlueKan+cysMPro

    Plasmid

    #187879

    Purpose

    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

    Depositor

    Gaylen Uhlich

    Article

    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CysM

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. psgRNA

    Plasmid

    #114005

    Purpose

    express sgRNA. ColE1, Kan

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pACYC-FLAG-dN6-His

    Plasmid

    #22143

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    Flag-clpXdeltaNLinkedHexamer-His6
    

    Use

    Tags

    Flag, His6, and L20 Linkers

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMA2775

    Plasmid

    #46884

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted myc-tagged E.coli exonuclease III

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    exonucleaseIII E. coli
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-Tight

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCis GFPuv-SS Himar1 A7

    Plasmid

    #84686

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pJLG038

    Plasmid

    #192981

    Purpose

    BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter

    Depositor

    Clay Fuqua

    Article

    Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge