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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,577 results for: nif

Showing: 1601 - 1620 of 2577 results

  2. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  3. pGP8G-ccdB

    Plasmid
    #83884
    Purpose
    Destination vector for integration at HIS3, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83884-plasmid-map-sequence-id-160280

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  4. pGP6G-ccdB

    Plasmid
    #83880
    Purpose
    Destination vector for integration at yeast URA3 locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83880-plasmid-map-sequence-id-160276

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  5. pGP4G-cccdB

    Plasmid
    #83876
    Purpose
    Destination vector for integration at yeast Trp locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83876-plasmid-map-sequence-id-160272

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  6. pGP5G-ccdB

    Plasmid
    #83879
    Purpose
    Destination vector for integration at yeast Leu locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83879-plasmid-map-sequence-id-160275

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  13. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  17. pOpen-Pwopol

    Plasmid
    #165562
    Purpose
    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Pwo DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165562-plasmid-map-sequence-id-340035

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  18. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

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  19. pOpen-T7gene1

    Plasmid
    #165505
    Purpose
    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T7 RNA Polymerase
    Use
    Synthetic Biology
    Available Since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165505-plasmid-map-sequence-id-338461

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  20. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    EcoRI
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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Showing: 1601 - 1620 of 2577 results