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pCMU-PLAr

Plasmid

#61200

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for mitochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Promoter

U6

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_RFP-TEVprotease

Plasmid

#124947

Purpose

Expression of RFP-TEVprotease

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-TEVprotease

Tags

mCherry

Expression

Mammalian

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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VSVGwt-SBP-EGFP_puromycin

Plasmid

#65302

Purpose

VSVG reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHnCBS1D

Plasmid

#52065

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D

Expression

Bacterial

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBAC-DsRed-QF2-hsp70

Plasmid

#104876

Purpose

General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

QF2

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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PB-Ostir-neo

Plasmid

#161973

Purpose

Expresses Ostir and G418 resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-neoR

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-MCS

Plasmid

#185594

Purpose

Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGFP-EB1

Plasmid

#17234

Depositor

Lynne Cassimeris

Article

Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

Insert

EB1 (MAPRE1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 11, 2008

Availability

Academic Institutions and Nonprofits only

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H2B-HT-mGold

Plasmid

#183988

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-lexAp65

Plasmid

#138393

Purpose

RMCE vector for generating C-terminal T2A-lexA-p65 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-lexAp65

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-tagRFP

Plasmid

#185599

Purpose

Lentiviral construct for expression of tagRFP-T targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-Ii

Plasmid

#65313

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Lentiviral

Mutation

Streptavidin in its cytoplasmic part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-Dre-T2A-TagRFP

Plasmid

#167968

Purpose

Transient expression of Dre and TagRFP

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

Dre recombinase

Tags

TagRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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