We narrowed to 7,693 results for: CCH

1. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87829

   Purpose

   High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   MTS
   NLS
   PTS1
   

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCWXPGR-pTF-dnTCF4

   Plasmid

   #114277

   Purpose

   TET-inducible expression of a dominant negative form of the TCF4 transcription factor

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Lentiviral

   Mutation

   deletion of AA 1 to 31

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZc-ScAct1*-thymosinB-8His

   Plasmid

   #111148

   Purpose

   Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   Act1 (ACT1 Budding Yeast)
   

   Use

   Pichia pastoris integration plasmid

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pATP416-­pluxO5-­pphlO6-­crtYBI

   Plasmid

   #165977

   Purpose

   Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pluxO5), Synthetic promoter (…

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-HA-mBec1 F121A

   Plasmid

   #99506

   Purpose

   Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

   Depositor

   Congcong He

   Article

   Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

   Insert

   Beclin 1 (Becn1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   F121A

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSH-pSUMO-α-COPI-WD40

   Plasmid

   #208341

   Purpose

   Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
   

   Tags

   His-tag, SUMO-tag, and Strep-tag

   Expression

   Bacterial

   Mutation

   Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ReactionC_pBIG1a_ATG7_ATG10

   Plasmid

   #190860

   Purpose

   Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG7 (ATG7 Human)
   ATG10 (ATG10 )
   

   Expression

   Bacterial and Insect

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid

    #165975

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. G30, Cdc20-mCherry-AID tagging

    Plasmid

    #130270

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SSH-pSUMO-β′-COPI-WD40-Lys17Ala

    Plasmid

    #208171

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
    

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Lys17Ala substitution

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

    Plasmid

    #229544

    Purpose

    Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    Oct4(co)-mNeonGreen (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Oct4-mNeonGreen

    Expression

    Mammalian

    Mutation

    codon-optimized OCT4

    Promoter

    TRE12

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid

    #208172

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid

    #208173

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

    Plasmid

    #208174

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCOA-Leu-FvPPT1-fsr1

    Plasmid

    #179868

    Purpose

    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Inserts

    PPT1
    fsr1
    

    Expression

    Yeast

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG1b_10xHisTEVATG5_ATG12

    Plasmid

    #190861

    Purpose

    Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG5 (ATG5 Human)
    ATG12 (ATG12 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial and Insect

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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