Addgene

We narrowed to 17,056 results for: URE

Showing: 1621 - 1640 of 17056 results

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-HH1713

Plasmid

#124994

Purpose

E. coli expression of recombinant H. hepaticus antigen HH_1713

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH_1713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Use

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01

Plasmid

#80046

Purpose

expression of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11 (1wa3)

Plasmid

#80122

Purpose

Palm1-Late1-I3-01-myc (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-Map7emtb-Kif5bmotor

Plasmid

#46077

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Kif5bmotor-Map7emtb

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Article

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Use

Tags

MBP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Use

Tags

his tag on Spc34 and strep tag on Dad1

Expression

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-1333-end

Plasmid

#46073

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1333-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-856-1351

Plasmid

#46072

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-856-1351

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-1783-end

Plasmid

#46074

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1783-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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mP14H1

Plasmid

#84369

Purpose

antigen for mouse PRDM14 purification ( aa 1-231)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14

Use

Tags

6xHis

Expression

Bacterial

Mutation

only aa 1-231 present

Promoter

T7

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Use

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18 (1wa3)

Plasmid

#80123

Purpose

PH-4GS-I3-01-myc-Late1 (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-25 (1wa3)

Plasmid

#80124

Purpose

I3-01p-myc-Late1-PH (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (1wa3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01(∆L1+∆L2)-His

Plasmid

#84511

Purpose

E.coli version of EPN01(∆L1+∆L2)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆L1+∆L2)-His

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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