164,811 results

1. FASTHDR-Cterm-mClover3-Puromycin

   Plasmid

   #167205

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. tFucci(CA)5pPBbsr2

   Plasmid

   #197351

   Purpose

   Encoding tFucci(CA)5.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   tFucci(CA)5
   

   Use

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229783

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-ChR2-GFP

   Plasmid

   #26929

   Purpose

   AAV-mediated expression of ChR2-GFP for neuronal excitation

   Depositor

   Edward Boyden

   Article

   Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

   Insert

   ChR2
   

   Use

   AAV; Adeno-associated virus

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Flpo

   Plasmid

   #125576

   Purpose

   Expresses Flpo under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   FLPo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-HTG

   Plasmid

   #27437

   Depositor

   Liqun Luo

   Article

   Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

   Insert

   histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
   

   Use

   AAV; Adeno-associated viral

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmRFP-LC3

   Plasmid

   #21075

   Purpose

   Mammalian expression of rat LC3 fused to mRFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB417

   Plasmid

   #74811

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SARS-CoV2 Nucleocapsid

    Plasmid

    #153201

    Purpose

    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

    Depositor

    Peter Klein

    Article

    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    

    Use

    Tags

    Myc tag (5X)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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