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Plasmid#229484PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k genesDepositorInsertE2A, VA RNA I, L4-22k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterCMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS-HsProm1s1(W795R)-StayGold
Plasmid#211334PurposeVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseAAVTagsStayGoldExpressionMutationChanged Trp-795 to ArgPromoterEF1aAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
Plasmid#207171Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expressionDepositorInsertGal4DBD-HIF1a-CAD Myc tag linker
UseTags2xMyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_R1683X
Plasmid#198111Purposelentiviral expression of hCOL4A4_R1683Xin mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationR1683XPromoterEF1aAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_C1684X
Plasmid#198112Purposelentiviral expression of hCOL4A4_C1684X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationC1684XPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_C29X
Plasmid#198107Purposelentiviral expression of hCOL4A5_C29X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationC29XPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_R1563X
Plasmid#198109Purposelentiviral expression of hCOL4A4_R1563Xin mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationR1563XPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_S36X
Plasmid#198108Purposelentiviral expression of hCOL4A4_S36X in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationS36XPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc
Plasmid#198106Purposelentiviral expression of hCOL4A4 in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsNlucExpressionMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1-AA027
Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1478
Plasmid#82424PurposePlasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cellsDepositorInsertnvGW182
UseTagsGSTExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1547
Plasmid#82434PurposePlasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cellsDepositorInsertnvAGO1
UseTagsHAExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1548
Plasmid#82435PurposePlasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cellsDepositorInsertnvAGO2
UseTagsHAExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD150
Plasmid#229485PurposeAdenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genesDepositorInsertE2A, VA RNA I, E4orf6, L4-22k/33k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterCMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_120
Plasmid#96917Purposefor CRISPRa, lentiviral expression of dCas9-VPR 2A BlastRDepositorInsertdCas9
UseLentiviralTagsVPRExpressionMammalianMutationD10A, N863APromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
JD171
Plasmid#229483PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genesDepositorInsertE2A, VA RNA I, L4-22k/33k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterCMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag
Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058APromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag
Plasmid#132400Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121APromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag
Plasmid#132407Purposeoverexpression in human cellsDepositorInsertdLwaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR474A; H479A; R1046A; R1051APromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag
Plasmid#132401Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only