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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCBL101.1

Plasmid

#199724

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

Multiple Cloning Site, NPTII

Expression

Plant

Mutation

"GAGACC" to "GATACC"

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSIM24-GUS

Plasmid

#67882

Purpose

Plant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

uidA

Expression

Plant

Mutation

T120A

Promoter

M24

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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p.UTA.3.0 Empty

Plasmid

#82447

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-SFNp-mRFP1

Plasmid

#238473

Purpose

Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

Depositor

Christopher Nelson

Article

Ivy et al BMC Methods. 2025;2(1):8. doi: 10.1186/s

Insert

SFNp-mRFP1

Promoter

SFNp

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pNCS-mRuby3

Plasmid

#74234

Purpose

Constitutive expression of mRuby3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mRuby3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-FLuc

Plasmid

#188311

Purpose

Expression of Firefly luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Firefly luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA

Plasmid

#138271

Purpose

Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

sgBG, sgTS

Use

CRISPR

Expression

Mammalian

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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CpxR-GFP

Plasmid

#220150

Purpose

To track the natural CpxR promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

CpxR promoter only

Expression

Bacterial

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBigT

Plasmid

#21270

Purpose

Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Type

Empty backbone

Use

Mouse Targeting

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pMPRAv3:∆luc:∆xbaI

Plasmid

#109035

Purpose

MPRA v3 empty backbone

Depositor

Pardis Sabeti, Ryan Tewhey

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Type

Empty backbone

Expression

Mammalian

Promoter

none

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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