We narrowed to 34,851 results for: CaS;

1. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBLO 9.3_bCas9-CP230

   Plasmid

   #121497

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Use

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTransposon.CAG.CasRX-P2A-Blast

   Plasmid

   #212965

   Purpose

   Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   CasRx
   

   Use

   Transposon

   Tags

   P2A-Blasticidin resistance

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas9-mD3A

   Plasmid

   #78257

   Purpose

   Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   E756A

   Promoter

   pCMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NG-YE1-BE4max

   Plasmid

   #198535

   Purpose

   Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-dCas9-GFP

    Plasmid

    #64106

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCVpuro-Cas9-EGFP

    Plasmid

    #159126

    Purpose

    Expresses active Cas9 fused with EGFP for fluorescence microscopy

    Depositor

    Taekjip Ha

    Article

    Liu et al Science. 2020 Jun 12;368(6496):1265-1269

    Insert

    Cas9 fused to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-opto-hCaspase-9

    Plasmid

    #208787

    Purpose

    Inducibly expresses human Caspase 8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase 9 (CASP9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-SaCas9-WPRE

    Plasmid

    #89996

    Purpose

    Expresses high levels of WT SaCas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SaCas9 (NEWENTRY Staphylococcus aureus)
    

    Use

    Tags

    3xHA, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.41

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-Myc-Caspase-5

    Plasmid

    #214305

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEM-Cas9HF1-recA56

    Plasmid

    #89962

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet and proD

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXT128_TwinStrep-SUMO-ShCas12k

    Plasmid

    #135524

    Purpose

    Protein expression construct for ShCast12k

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShCas12k
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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