We narrowed to 3,211 results for: beta-actin;
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Viral Prep#179255-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV.CAG.FLEX.iGluSnFR3.v857.GPI
Plasmid#196218PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG.FLEX iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-eYFP (AAV PHP.eB)
Viral Prep#104055-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-eYFP (#104055). In addition to the viral particles, you will also receive purified pAAV-CAG-eYFP plasmid DNA. CAG-driven eYFP expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEYFPAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mScarlet-Giantin
Plasmid#196872PurposeExpresion of Giantin-fused to mScarlet. Used as Golgi markerDepositorAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG PSMA nanobody T-CAR
Plasmid#171962PurposeDonor plasmid for PSMA nanobody T-CAR expression in human cellsDepositorInsertSynthetic anti-PSMA nanobody CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS
Plasmid#199252PurposeExpression construct encoding the high specificity eSpCas9(1.1)-D10A nickaseDepositorInsertHigh specificity eSpCas9(1.1)-D10A nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_Puro
Plasmid#134845PurposeAll in one overexpression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPRTagsT2A PuromycinExpressionMammalianPromoterCAGAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5-4x6T
Plasmid#196419PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV and Cre/Lox; Astrocyte-selectiveTagsLckExpressionMammalianPromoterCAGAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-3xGFP
Plasmid#191204PurposeFlpO dependent GFP expressionDepositorInsert3X GFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EBFP2-P2A-EGFP
Plasmid#184938PurposeExpresses blue fluorescent protein EBFP2 and EGFP in mammalian cellsDepositorInsertEBFP2-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-lung (UPB2)
Plasmid#180788PurposeUnique Projection Barcode (UPB2)-Lung for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVExpressionMammalianPromoterCAGAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-IRES2-p75NTR-GFP
Plasmid#182947PurposeTo express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-TerminalDepositorInsertp75NTR-mGFP
TagsHA tag and mGFPExpressionMammalianMutationThe leader sequence is from preprotrypinPromoterCAGAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ArcLight-ST
Plasmid#182318PurposeFluorescent voltage indicator with left-shifted voltage-dependencyDepositorInsertArcLight-ST
ExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-Antares2
Plasmid#120868PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertpcDNA3-Antares2
ExpressionMammalianMutationAntares2 ampliconPromoterCAG PromoterAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-mCherry
Plasmid#170519PurposePlasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-mCherry
UsePiggybac transposonExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-CIP4
Plasmid#178363PurposeExpresses CIP4 c-terminal labeled with EGFP in mammalian cellsDepositorAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only