We narrowed to 4,840 results for: donor

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qTAG-C-dTAG-Zeo

Plasmid

#207738

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Publication

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR-53BP1 1-1972 28A

Plasmid

#53447

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway Cloning

Mutation

I914T mutation; All 28 SQ/TQ motifs in N-term of …

Available since

Dec. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSLHDR02_EGFP_tCD8

Plasmid

#124061

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Publication

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

EGFP
tCD8

Use

Mouse Targeting; Human targeting

Promoter

Human UbC promoter modified

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Publication

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCyRFP1-Cdc42

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-PKCb1-mEGFP-N1

Plasmid

#112265

Purpose

ITRACKbeta/IDOCKSbeta Donor construct

Depositor

Ryohei Yasuda

Publication

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

protein kinase c beta (Prkcb Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1825_pCR8-dCasRx-RBM38

Plasmid

#118662

Purpose

Gateway Donor; dCasRx-RBM38 ORF; No promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro

Plasmid

#227253

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-dTAG

Plasmid

#207683

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Publication

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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