We narrowed to 5,839 results for: pCas

1. pCAGGS-SARS2-S-G614 (C-flag)

   Plasmid

   #156421

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-RFP-p2a-postASAP

   Plasmid

   #178793

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with RFP

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and TagRFP
   

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hChR2(H134R)-EYFP

   Plasmid

   #114367

   Purpose

   expresses channelrhodopsin 2 fused with EYFP

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   hChR2(H134R)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   the Histidine at amino acid position 134 was chan…

   Promoter

   pCAG

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-1BPNLS-Cas9-1BPNLS

   Plasmid

   #87108

   Purpose

   BPNLS-Cas9-BPNLS expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-EcRT-GAL-HIS3

   Plasmid

   #232093

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-EcRT-GAL-Nat

   Plasmid

   #232090

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9-EcRT-GAL-Kan

   Plasmid

   #232092

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-LplA(AILR,36)-NRX3b

   Plasmid

   #43919

   Depositor

   Alice Ting

   Article

   Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

   Insert

   AILR-LplA36-NRX3b (lplA Human, Synthetic)
   

   Tags

   2XHA

   Expression

   Mammalian

   Promoter

   Chicken beta actin

   Available Since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS/XBB.1.16-RBD

    Plasmid

    #213070

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/XBB.1.16-RBD (S )
    

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-pac-T2A-iCre (v2)

    Plasmid

    #125821

    Purpose

    Puromycin resistance gene and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

    Plasmid

    #141048

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF1-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_GST-TEV-EGFP-ATG13

    Plasmid

    #223797

    Purpose

    Expression of recombinant protein for purification

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST-TEV-EGFP

    Expression

    Mammalian

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-VSFP Butterfly 1.2

    Plasmid

    #47978

    Purpose

    Genetically encoded voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

    Insert

    VSFP Butterfly 1.2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAX APPs-695-alpha

    Plasmid

    #30147

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein - soluble (APP Human)
    

    Expression

    Mammalian

    Mutation

    amino acids 1–612

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-sfGFP-iCre-ERT2

    Plasmid

    #125749

    Purpose

    sfGFP and iCreERT2 fused by unskippable linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-pac-T2A-FLPo

    Plasmid

    #124835

    Purpose

    Puromycin resistance gene and FLP recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-FLPo
    

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS D614G soluble Spike

    Plasmid

    #164651

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only