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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,124 results for: Met

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Showing: 1681 - 1700 of 10124 results

  8. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Publication
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  9. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid
    #176534
    Purpose
    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:
    Insert
    CRISPR-associated IscB (Delaware Bay metagenome)
    Tags
    14xHis-MBP-bdSUMO and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    176534-plasmid-map-sequence-id-345508

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  10. SUV39H2

    Plasmid
    #25115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H2_04 (SUV39H2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  11. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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  16. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid
    #119723
    Purpose
    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
    Depositor
    Thomas Oertner
    Publication
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Inserts
    CaMPARI2
    CaMPARI2
    Use
    AAV
    Tags
    NES-his
    Expression
    Mammalian
    Mutation
    F391W, L398V
    Promoter
    hSyn1
    Available since
    Jan. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    119723-plasmid-map-sequence-id-232115

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Showing: 1681 - 1700 of 10124 results