We narrowed to 3,370 results for: actin

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pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF5B

Plasmid

#134625

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCI H2B-GFP

Plasmid

#92399

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-FilaminA-N-9

Plasmid

#57379

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

FilaminA (FLNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUB_smFLAG_ActB_MS2

Plasmid

#81083

Purpose

Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_ActB_24xMS2

Expression

Mammalian

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

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mKate1.31-Lifeact-7

Plasmid

#54668

Purpose

Localization: Actin, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

LifeAct

Tags

mKate1.31 (aka mKate2)

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mEos4b-Lifeact-7

Plasmid

#54813

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

Lifeact-mEos4b

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pIS1-Actb5UTR-renilla

Plasmid

#38234

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Actb 5'UTR / Renilla (Actb Mouse)

Expression

Mammalian

Mutation

Actb 5' UTR fused to renilla ORF

Promoter

Endogenous Actb

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-memRFP-3xPax7gRNA

Plasmid

#224569

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

CRISPR

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

Plasmid

#53959

Purpose

allows Cre-mediated expression of transgene

Depositor

Jeffrey Robbins

Article

Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

Type

Empty backbone

Use

Cre/Lox

Tags

Lox-CAT-Lox

Expression

Mammalian

Promoter

CMV enhancer, chicken actin promoter

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab5a

Plasmid

#64209

Purpose

Rab5a with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab5a (Rab5a Mouse)

Tags

3myc and FRB

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lifeact-μDpTT

Plasmid

#182322

Purpose

Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lifeact-μDronpa-TagRFP-T

Tags

N-terminal 17 amino acids from budding yeast Abp1…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pB.FRB-3myc-KLC1a

Plasmid

#134627

Purpose

Mammalian expression of mouse KLC1a

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KLC1a (Klc1 Mouse)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CAP Z alpha 1 beta 1 CP

Plasmid

#13451

Depositor

John Cooper

Article

Soeno et al J Muscle Res Cell Motil. 1998 Aug . 19(6

Insert

capping protein alpha 1 beta 1 (chicken) (CAPZB Chicken)

Expression

Bacterial

Mutation

none

Available Since

Dec. 22, 2006

Availability

Academic Institutions and Nonprofits only
pR008_APAD

Plasmid

#204818

Purpose

Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

Depositor

Xiaochang Zhang

Article

Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

Insert

rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)

Tags

HA and Flag and huADAR2dd

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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miniSOG-Lifeact-7

Plasmid

#54823

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-miniSOG

Tags

Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits