We narrowed to 11,199 results for: NSI

1. pPOTv7-blast-10FLAG

   Plasmid

   #181940

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   10xFLAG
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LV anti-FITC-LaG16-R103A-T2A-BFP

   Plasmid

   #194935

   Purpose

   LV construct encoding R103A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-Chronos-mRuby2-ST

   Plasmid

   #105446

   Purpose

   Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Insert

   Chronos-mRuby2-ST
   

   Use

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM705 Gst (Cys)

   Plasmid

   #109376

   Purpose

   GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gst chimera
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Cys at position 391 (Pertussis Toxin Sensitive)

   Promoter

   CAG

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-1b-S2

   Plasmid

   #128590

   Purpose

   Expresses tagless S2 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S2 (rpsB E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLAG-CMV2-CARP2

   Plasmid

   #16013

   Depositor

   Wafik El-Deiry

   Article

   McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

   Insert

   CARP2 (RFFL Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pRYG

   Plasmid

   #113642

   Purpose

   Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. slc6a3 probe

   Plasmid

   #53764

   Purpose

   cRNA probe to detect the slc6a3 transcript

   Depositor

   Edward Burton

   Article

   Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

   Insert

   DAT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Y-RAN-RFP6

   Plasmid

   #106424

   Purpose

   Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

   Insert

   Y-RAN-RFP6
   

   Use

   Tags

   HA and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK240

    Plasmid

    #71127

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    GyrA intein and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV TRE-GFP-TMD-DLL1

    Plasmid

    #194936

    Purpose

    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG.3xHalo-GFP-SPC25

    Plasmid

    #176206

    Purpose

    Kinetochore anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    3xHalo-GFP-SPC25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_L706-antiSH2-OptoMB-irFP

    Plasmid

    #159938

    Purpose

    Opto-Monobody mammalian expression

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKDH

    Plasmid

    #67584

    Purpose

    Gene expression in plants. A promoter and/or reporter can be cloned into this plasmid

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    None

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-HA-mAVO3 (pRL62-3)

    Plasmid

    #39210

    Depositor

    Michael Hall

    Article

    Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Ep

    Insert

    mAVO3 (Rictor Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G5S and I15V

    Promoter

    CMV

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pSW549 YFP/mVenus

    Plasmid

    #115984

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    mVenus
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-RA-HRas-IRES2-Lyn-iRFP682

    Plasmid

    #180584

    Purpose

    RA-Hras and Lyn-iRFP682

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    RA-Hras and Lyn-iRFP682
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-KG-GST-YAP-S127A

    Plasmid

    #33053

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pMC-1-PvUbi2-6

    Plasmid

    #197706

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    PvUbi2 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMC-1-AtUbi10-6

    Plasmid

    #197700

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtUbi10 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMC-X4

    Plasmid

    #176154

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMC-XII1

    Plasmid

    #176159

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5_tAKARα-T391A

    Plasmid

    #119920

    Purpose

    Mammalian expression of the tAKARα sensor with T391A mutation

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα-T391A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMC-XI3

    Plasmid

    #176157

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSETA_crE6G2

    Plasmid

    #172929

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6G2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Val317Gly, Met316Gly

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-mWnt9b

    Plasmid

    #42287

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Wnt9b (Wnt9b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pegRNA_LRRK2-3

    Plasmid

    #199272

    Purpose

    pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LRRK2 6055GtoA pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMC-XII5

    Plasmid

    #176161

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFLAG-CMV2-CARP1

    Plasmid

    #16014

    Depositor

    Wafik El-Deiry

    Article

    McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

    Insert

    CARP1 (RNF34 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pExo1

    Plasmid

    #53357

    Purpose

    Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

    Depositor

    Lingchong You

    Article

    Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

    Inserts

    BlaMs
    hlyAs
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p(lac/ara-1)

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pL-rpb1-E1103G

    Plasmid

    #10989

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-E1103G (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 3308 A to G in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pET14b_SG

    Plasmid

    #59737

    Purpose

    Expresses SH3-GUK domains of human ZO-1

    Depositor

    Alan Fanning

    Article

    Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EGFP-PH-dL5**

    Plasmid

    #96947

    Purpose

    Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    EGFP and dL5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PH-dL5**

    Plasmid

    #96946

    Purpose

    PLC pleckstrin homology domain linked to FAP for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    dL5 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJin-278

    Plasmid

    #125714

    Purpose

    ABA-inducible split T7 GFP vector using RNAPN d5-19

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPOTv6-blast-3Myc:HaloTag:3Myc

    Plasmid

    #181943

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    HaloTag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPOTv7-blast-mCherry

    Plasmid

    #179789

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR-TIR1

    Plasmid

    #83875

    Purpose

    Gateway donor vector for TIR1

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. UAS-Syt::3xHalo7

    Plasmid

    #87649

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::3xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::3xHalo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge