Addgene

We narrowed to 15,322 results for: ef1

Showing: 1701 - 1720 of 15322 results

Results per page:

Sort results by:

pAAV-EF1α1.1-tdTomato

Plasmid

#122101

Purpose

AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

tdTomato

Use

AAV

Tags

NA

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_EF1a-->sGFP_PGK-->puroR

Plasmid

#162156

Purpose

Lentiviral vector for constitutive expression of GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
R777-E223 Hs.RAPGEF1

Plasmid

#70507

Purpose

Gateway ORF clone of human RAPGEF1 [NM_198679.1] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RAPGEF1 (RAPGEF1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS(A) mLef1 (CT#1141)

Plasmid

#14021

Depositor

Cliff Tabin

Insert

Lef1 (Lef1 Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pLVH-EF1A-BRD3R

Plasmid

#130696

Purpose

Lentiviral plasmids encoding human BRD3R

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

Human BRD3R (BRD3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1A-CCNE1

Plasmid

#162553

Purpose

Overexpression of CCNE1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE1 (CCNE1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a_CD MUT TET1

Plasmid

#124083

Purpose

Expression of mutated catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - catalytic domain mutated (TET1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

H1672Y, D1674A

Promoter

EF1 alpha

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EF1aL-nlsGFP-W

Plasmid

#126688

Purpose

Constitutive, ubiquitous, nuclear-localized GFP expression.

Depositor

Darrell Kotton

Article

Tang et al Nat. Phys. 18, 1371–1378 (2022).

Insert

nlsGFP

Use

Lentiviral

Tags

6xHIS and NLS

Expression

Mutation

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-N-cG-Blast

Plasmid

#124981

Purpose

Express Nanoluc, copGFP

Depositor

Kazuhiro Oka

Insert

Nanoluc, copGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Fast-GCaMP-EF16

Plasmid

#52642

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF16

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N/E442A mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF19

Plasmid

#52644

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF19

Use

Tags

Expression

Mammalian

Mutation

T328D/T330D mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α1.1-GFP

Plasmid

#122100

Purpose

AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

GFP

Use

AAV

Tags

NA

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS505 EF1alpha-EGFPcre*

Plasmid

#11955

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shLEF1 a

Plasmid

#27019

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 3a

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge