Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,966 results for: rigi

Clear keywords
Showing: 1701 - 1720 of 1966 results

  1. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PR promoter and PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

    Enlarge

  2. pTA1cI-T3

    Plasmid
    #193789
    Purpose
    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193789-plasmid-map-sequence-id-389812

    Enlarge

  3. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    David Andrews
    Insert
    Bcl-2-interacting killer (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191411-plasmid-map-sequence-id-376884

    Enlarge

  4. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

    Enlarge

  6. pSTAP-seq_fly-zfh1_spikeIn-1

    Plasmid
    #86387
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
    Use
    Stap-seq spike in vector
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86387-plasmid-map-sequence-id-193668

    Enlarge

  7. pSTAP-seq_fly-zfh1_spikeIn-3

    Plasmid
    #86389
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
    Use
    Stap-seq spike in vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86389-plasmid-map-sequence-id-173956

    Enlarge

  8. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid
    #86390
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    Use
    Stap-seq spike in vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86390-plasmid-map-sequence-id-173960

    Enlarge

  9. pSTAP-seq_fly-zfh1_spikeIn-2

    Plasmid
    #86388
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
    Use
    Stap-seq spike in vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86388-plasmid-map-sequence-id-173955

    Enlarge

  12. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

    Enlarge

  13. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

    Enlarge

  14. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

    Enlarge

  15. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

    Enlarge

  17. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

    Enlarge

Showing: 1701 - 1720 of 1966 results