We narrowed to 1,854 results for: rigi

1. pTH726-CEN-RLuc/minCFLuc

   Plasmid

   #38210

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY152

   Plasmid

   #130950

   Purpose

   dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   PpspA-2G6 and Ptet

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. RH2b-bio

   Plasmid

   #47778

   Purpose

   Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH2b (partial)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pf92-bio

   Plasmid

   #47728

   Purpose

   Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised Pf92
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH744-CEN-RLuc/slowmaxCFLuc

   Plasmid

   #38224

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RON6-bio

   Plasmid

   #47785

   Purpose

   Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RON6
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin replaced…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH742-CEN-RLuc/slowminCFLuc

   Plasmid

   #38222

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET SUMO-Doc2β C2AB (G361C)

   Plasmid

   #213631

   Purpose

   Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   Doc2β C2AB (G361C) (Doc2b Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   G361C, all original cysteines mutated to alanines

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUGP1.1

   Plasmid

   #196615

   Purpose

   Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CLC-EGFP206(5)

    Plasmid

    #193573

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 207-211

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLC-EGFP206(6)

    Plasmid

    #193574

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 207-211

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CLC-EGFP206(7)

    Plasmid

    #193575

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 207-211

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. vPyACR_2164382

    Plasmid

    #153029

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    vPyACR_2164382
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    vPyACR_2164382 was C-terminally truncated to incl…

    Promoter

    CMV (+ enhancer)

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. vPyACR_21821

    Plasmid

    #153030

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    vPyACR_21821
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    vPyACR_21821 was C-terminally truncated to includ…

    Promoter

    CMV (+ enhancer)

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pC-G418-YR

    Plasmid

    #61767

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid

    #191097

    Purpose

    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-vsfGFP-0
    

    Use

    AAV

    Tags

    Green fluorescent protein bound to enhancer nanob…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge