We narrowed to 21,797 results for: cas9

1. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBECKP-km

   Plasmid

   #117235

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_antiCD19_H1_Gal4VP64

   Plasmid

   #169918

   Purpose

   Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch1-GL4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh141

   Plasmid

   #106811

   Purpose

   expression of gRNA targeting OGT

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   OGT (OGT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xyl/tet promoter that is constitutively active

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE LOX Cloning template vector

   Plasmid

   #139651

   Purpose

   Cloning template for ORANGE-CAKE conditional knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Blasticidin-CAG-Flpe-ERT2

   Plasmid

   #68461

   Purpose

   AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   mammalian-codon-optimized Flpe recombinase fused with ERT2
   

   Use

   Synthetic Biology

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_053

   Plasmid

   #113591

   Purpose

   Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

   Insert

   sgRNA cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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