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Showing: 1721 - 1740 of 7046 results

  1. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid
    #229824
    Purpose
    A PiggyBac vector with a cre dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Luciferase-P2A-H2A-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229824-plasmid-map-sequence-id-460485

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  7. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225649-plasmid-map-sequence-id-448716

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  8. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsine promoter and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177360-plasmid-map-sequence-id-353669

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  9. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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  13. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid
    #194608
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Deletion of N-terminal CARD domain and changed Cy…
    Promoter
    CMV
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194608-plasmid-map-sequence-id-384891

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  16. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193318
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193318-plasmid-map-sequence-id-383635

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  17. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

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  18. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid
    #186710
    Purpose
    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.
    Depositor
    Paula Tamagnini
    Article
    Mascia et al Green Chemistry, 24 (2022):6156
    Insert
    alr4766
    Use
    Synthetic Biology
    Tags
    His-Tag (6His)
    Expression
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    186710-plasmid-map-sequence-id-368444

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  20. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid
    #136884
    Purpose
    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
    Depositor
    Moritz Mall
    Article
    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi
    Insert
    engrailed repressor domain (en Fly)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    136884-plasmid-map-sequence-id-272359

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Showing: 1721 - 1740 of 7046 results