We narrowed to 2,033 results for: endog

1. pRPL28(Q38P) SpHIS5 (pNTI664)

   Plasmid

   #115434

   Purpose

   Expresses Q38P RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Q38P; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPL28(H39L) SpHIS5 (pNTI665)

   Plasmid

   #115435

   Purpose

   Expresses H39L RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H39L; MfeI site in intron

   Promoter

   ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS315Nab3D191stop

   Plasmid

   #110537

   Purpose

   Expresses yeast NAB3 using the endogenous promoter

   Depositor

   Daniel Reines

   Article

   Loya et al PLoS One. 2017 Oct 12;12(10):e0186187. d

   Insert

   NAB3 (NAB3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal deltion of 191 amino acids

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GTU-c

   Plasmid

   #81093

   Purpose

   Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   yemCFP
   

   Use

   Cre/Lox

   Tags

   yemCFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. GTU-y

   Plasmid

   #81095

   Purpose

   Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCitrine
   

   Use

   Cre/Lox

   Tags

   mCitrine

   Expression

   Yeast

   Mutation

   Mutated mCitrine for monomeric isoform (A206K L22…

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GTU-ir

   Plasmid

   #81097

   Purpose

   Gene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   tdiRFP
   

   Use

   Cre/Lox

   Tags

   tdiRFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GTL-c

   Plasmid

   #81098

   Purpose

   Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   yemCFP
   

   Use

   Cre/Lox

   Tags

   yemCFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GTT-c

   Plasmid

   #81108

   Purpose

   Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   yemCFP
   

   Use

   Cre/Lox

   Tags

   yemCFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. miR-451 Trap

   Plasmid

   #61487

   Purpose

   to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   miR-451 Trap
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional phosphoglycerate kinase promoter (b…

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAC-BETA

    Plasmid

    #53272

    Purpose

    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

    Insert

    crtE, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSNR52-sgTEF1

    Plasmid

    #46922

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgTEF1 promoter (TEF1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Use

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-S5E2-FingR-PSD95-mGL

    Plasmid

    #217533

    Purpose

    Genetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    ZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    Promoter

    S5E2 enhancer

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. RepairTemplate_NIPBL_mEGFP_mAID

    Plasmid

    #217664

    Purpose

    Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    NIPBL_mEGFP_mAID (NIPBL Human)
    

    Use

    Tags

    mAID and meGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid

    #86843

    Purpose

    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

    Depositor

    Thomas Cech

    Article

    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

    Insert

    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    

    Use

    Tags

    FLAG-HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous TERT promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP777_SnoopCatcher-p65HSF1-CO

    Plasmid

    #211770

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-p65HSF1
    

    Use

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LLP775_αGCN4-p65HSF1-CO

    Plasmid

    #211768

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-p65HSF1
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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